New method identifies eight proteins in one cell using fluorescence timing
An international team led by the University Medical Center Göttingen has developed a technique to visualize eight different proteins simultaneously in a single staining step.
Standard microscopes have color limits
Conventional fluorescence microscopy typically allows researchers to detect only three or four colors at once. This limitation means scientists can distinguish only that many proteins when using standard equipment. The new approach overcomes this barrier by measuring how long a dye takes to emit light instead of relying on color differences.
Single step labels multiple targets
The developed labeling method distinguishes fluorescent markers based on their emission timing rather than their hue. Researchers can now visualize eight different proteins in a single cell using just one staining procedure. This process utilizes standard microscopes without requiring specialized hardware upgrades for the detection system.
Results published in ACS Nano
The findings were recently published in the journal ACS Nano by an international collaboration. Dr Felipe Opazo led the research group at the University Medical Center Göttingen during this project. The study appears alongside work from Dr Roman Tsukanov at the Third Institute of Physics.
Reported by one outlet
Only one outlet has published this. Nothing here has been checked against a second report, so read it as that outlet's account and follow the link below for the original.
Reported by
1 independent outlet. Headline as published. Links open the original report.